Study of Different Mutation Types of HVI Region and TSPY1 Copy Number in Different Nationalism of Kirkuk City

Abstract

Geographical barriers have been recognized as significant impediments to population mobility, and these barriers may have varying effects on various genetic markers, including mitochondrial DNA (mtDNA) and the Y chromosome. Therefore, the current study aimed to detect a different types of mutations of HVI and TSPY1 copy number in different Nationalism of Kirkuk city. The present investigation was carried out at the University of Kirkuk's Department of Life Sciences, College of Science, molecular laboratory. Blood samples were taken in November 2022 from 500 people in the Kirkuk governorate, 300 of whom were male and 250 of whom were female. This included unrelated people. Data and samples are collected in accordance with environmental ethics. The results were found that 16 mutations were of the Transition type, with C\T being the highest, accounting for 16.8% of the total mutations. While the number of Transversion mutations was 33, with A\T being the highest, accounting for 8.7% of the total mutations. Deletion and Insertion mutations were also found, especially T addition, accounting for 20.1%, and C deletion, accounting for 5.4%. The median TSPY1 copy number of original Arabs of Kirkuk was 1717.224+450.30, Kakai was 4220.5+187.57, Original Kurds of Kirkuk was 3278.8+755.62, Turkmen of Kirkuk was 2359.25+756.71, Christian was 4885.8+76.57 and also the median TSPY1copy number of other nationalism was appaered in table (2). the median TSPY1copy number of all kirkuk nationalism did not differ significantly Kruskal-Wallis test (p = 4.05). its concluded that more variations of the TSPY1 copy number was observed in case of Kakai and Christian compared to other nationalism.