Synthesis of Gold Nanoparticles from Candida Dubliniensis for use as an Anti-Bacterial Agent against some Bacteria-Caused Vaginal Infections

Abstract

This work in our paper aimed to discuss the synthesis in extracellular of gold nanoparticles (AuNPs) by utilizing the supernatant of the yeast Candida dubliniensis, which was recluse from the oral mucosa of patients wearing removable dentures and identified through molecular assays. Various techniques were used to characterize the AuNPs. The medium's color shifted (light yellow-dark violet), and a sucking peak was detected at wavelength 550 nm utilizing ultraviolet (UV)-visible spectroscopy. Scanning electron microscopy (SEM) uncovered that the AuNPs were spherical, with sizes ranging from 58.07 to 72.15 nm. Additionally, Fourier Transform Infrared (FT-IR) spectroscopy was utilized to distinguish active functional groups. Gold nanoparticles have been evaluated for antibacterial activity against human pathogenic bacteria isolated from Vaginitis infections, including Staphylococcus aureus, Staphylococcus haemolyticus, Escherichia coli, Klebsiella sp., and Micrococcus sp. On Muller Hinton agar plates, the activity of anti-bacterial was assessed utilizing the agar well diffusion technique with concentrations ranging from 31.25 to 500 µg of AuNPs. The produced gold nanoparticles demonstrated variable growth inhibition activity (1-23 mm) inhibitory zones against the assessed harmful bacteria.