The Skin Wounds and Effects of Epicyn, Flosteron and Contratubex on il-1 and il-6 Cytokines In the Dynamic of Healing Process

Abstract

Skin wounds, ranging from minor cuts to severe injuries, are common occurrences in everyday life. The problem of wound healing is multifaceted, involving scar formation, infection risk, and cosmetic outcomes.  The wound healing is a complex, dynamic and highly regulated process involving a cascade of cellular and molecular events. Interleukin-6 and interleukin-1 emerge as critical mediators in orchestrating inflammation and tissue repair during wound healing. Understanding the roles of these cytokines provides valuable insights into the mechanisms underlying wound healing and may offer potential therapeutic targets for enhancing the healing process in various pathological conditions. Presented article provides results of investigation carried out on lab. rats designed to study effects of Flosteron, Contratubex and Epicyn on IL-1 an IL-6  cytokines during the skin wound healing process. Excisional, full-thickness skin wounds were aseptically made on the dorsal skin of lab. rats. Contractubex and Epicin creams were applied to the wound surface as a thin layer 2-3 times a day for 4 weeks, Flosteron (0.2 ml) was injected subcutaneously in the wound area once a week during 4 weeks.  Proinflammatory cytokines (IL-1, IL-6) were studied by ELISA.  Results showed that IL-1 on the 7^th day of wound healing was increased significantly in all experimental group animals compared to the data of healthy group animals. In subsequent days the IL-1 gradual decrease was detected only in the epycin-treated group animals and by the 21^st day of wound healing it was within the normal range. IL-1 in flosteron-treated animals was normalized by 28^th day of wound healing, while in control and contratubex-treated animals it was still increased by 35% and 24% respectively. Increase in IL-6 was detected later, on the 14^th day of wound healing.  Its gradual decrease and normalization was obvious in epicyn- and flosteron-treated animals on the 28^th day. There was no significant difference in data of control and contratubex-treated animals and by the 28^th day of healing process IL-6 was still increased by 17% and 14% respectively compared to normal value.  Conclusion: IL-1 and IL-6 are pivotal in skin wound healing, with implications for inflammation modulation and tissue repair. During wound healing, IL-1 and IL-6 exhibit distinct temporal patterns, reflecting their roles in different phases. As wounds progress, IL-1 and IL-6 decline, signaling resolution of inflammation and tissue remodeling. Although, epicyn revealed better wound healing properties, further research is warranted to elucidate the precise mechanisms underlying the effects of flosteron,  contratubex, and epicyn on IL-1 and IL-6 dynamics during wound healing.  Therapeutic interventions targeting these cytokines may offer promising avenues for enhancing wound healing outcomes.